Reporter

Part:BBa_K5316020:Design

Designed by: Santiago Ramos Bartolome   Group: iGEM24_Barcelona-UB   (2024-09-30)


H2B-mCherry


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 189
    Illegal PstI site found at 736
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 189
    Illegal PstI site found at 736
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 189
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 189
    Illegal PstI site found at 736
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 189
    Illegal PstI site found at 736
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Used an additional Kozak before mCherry to promote proper folding.


Source

H2B: BBa_K53160007

Kozak: BBa_K4046050

mCherry: BBa_K4839013


References